Chromatography, Advanced Course

Techniques of, mostly chromatography-related, modern analytical separation and quantification methods are presented. Topics presented are a.o. chromatographic theory including the solvation parameter model of retention; some advanced aspects of liquid chromatography (HPLC) and gas chromatography (GC); 2-dimensional chromatography (coupled methods chromatography-chromatography); 'tandem techniques' based on on-line coupling of separation methods and spectrometry; mass spectrometry, with focus on applications in connection with analytical separation methods; Other relevant topics can be included, such as capillary electromigration separation methods, supercritical fluid chromatography, stereoisomer separation and preparative chromatography techniques. Students choose one of them for self-studying, as an elective part of the final examination. Laboratory exercises apply some of these concepts in a conceptual learning framework. Exercises provide the possibility for some repetition of basic concepts, discussions of relevant applications from current research papers and presentation of special topics of current research interests. This course has nearly the same content, and is taught together with, KJ8059.

At the end of the course a student can:

• explain the principles of a wide range of modern analytical methods of separation and quantitation;
• apply the methods in a sustainable context such as circular economy;
• evaluate strengths and limitations of different separation and detection techniques with respect to sample properties and to specific analytical problems;
• choose and plan the use of suitable analytical separation techniques for actual analytical problems based on a sound knowledge of the potentially relevant methods;
• judge the quality of his / her own, or others', analytical results from analytical separation methods, and to evaluate the chosen operational procedures;
• understand and if necessary adapt to own requirements, published procedures, eg to locally available technical resources or to more modern practices;
• apply analytical methods of separation and quantitation in an effective and reasonable way after only a minimum of practical teaching;
• consider HSE in relation to chromatographic experimentation.

Lectures (3 hours per week), exercises (1 hour per week), and laboratory exercises (40 hours). All laboratory exercise reports must be approved in order to get access to the final examination. Schedule will be published at semester start. Expected work load in the course is 200-225 hours.