MOL3010
Animalsk cellekultur
Sist undervist 2019
Høst
Trondheim
Engelsk
Om emnet
Faglig innhold
The course will focus on practical aspects of cell culture, like design and layout of the laboratory, aseptic technique, cloning and selection of specific cell types, contamination, methods for measuring viability and cytotoxicity, cell culture environment (substrate, gas phase, medium), and the culturing of specific cell types.
Læringsmål
After completing the course MOL3010, the student shall be able to:
- demonstrate knowledge of basic cell culture techniques
- demonstrate knowledge of establishment of cell inlines and their maintenance
- demonstrate knowledge on design and use the cell culture facilities
- critically evaluate cell cultures constraints and possibilities as an in vitro model
- discuss the advantages and limitations of primary cell culture compared to immortalized or transformed cell lines.
Læringsformer og aktiviteter
Self-tuition.
The language of examination is English.