Functional Genomics: Current Problems and Methods

The course contains a) introduction to functional genmics and b) MOL 8003. Fundamental principles within functional genomics, emphasizing the transcriptome and the proteome. Microarray-technology (production, different formats, isolation and labelling of samples, hybridisation, image analysis).
Protein separation (2D-PAGE, 2D-LC). Mass spectrometry (MALDI-TOF, ESI-MS). Protein structure analysis (X-ray chrystallography, NMR). Experimental design, data preprocessing and -analysis. Ethical perspectives within functional genomics and genetic risk information.

The students shall gain insight into fundamental principles within functional genomics and how this area of research contributes to development of new knowledge in basic biomedical science as well as to development of improved diagnostics and treatment of disease. The students shall also be able to understand the interplay of biomedicine, bioinformatics and bioethics within this field of research and be able to design and carry out studies within functional genomics based on microarray and mass spectrometry.

Lectures, laboratory course.